Sample Submission & Requirements

Please submit samples to the basement of the main building, in front of the room 33. The samples must be labeled and accompanied by a submission form (Download: Adobe PDF or Microsoft Word). Please make a record of each sample in a log book located outside the room 33. A freezer (-12° C) is available for unstable samples. In case of toxic or otherwise hazardous samples please contact the laboratory staff. The samples that are not related to the projects currently running in the IOCB and radioactive samples cannot be accepted!

Mass spectra will be saved in pdf format and uploaded to the local network. Unless stated otherwise in the submission form the samples will be discarded.

Sample requirements:

APCI: The sample (10 µg - 1.0 mg) should be submitted as the dry solid or the neat liquid in a vial. The sample must be soluble in a suitable solvent compatible with APCI. The best solvents are methanol, acetonitrile, ethanol, 2-propanol, or acetone.

EI: The sample (100 µg - 1.0 mg) should be submitted as the dry solid or the neat liquid in a vial. The samples must have sufficient volatility and thermal stability to evaporate from the sample probe under vacuum. The samples for GC/MS must be free from nonvolatile buffers, strong acid, bases and oxidizing compounds.

ESI: The sample (10 µg - 1.0 mg) should be submitted as the dry solid or dissolved in a suitable solvent compatible with electrospray. The best solvents are methanol, acetonitrile, and their mixtures with water. The samples must be free from nonvolatile buffers, solvents, and surfactants.

MALDI: The sample (10 µg - 1.0 mg) should be submitted as the dry solid or in a solution free from nonvolatile buffers, solvents, and surfactants. Proteomic samples are submitted as gels or tryptic digests (recommended protocol).