Baird, J. H., Kopecký, D., Lukaszewski, A.J., Green, R.L., Bartoš, J., Doležel, J.
CROP SCIENCE
52:
408-412,
2012
Keywords:
DArT, Diversity Arrays Technology; NTEP, National Turfgrass Evaluation Program; RFLP, restriction fragment length polymorphism
Abstract:
Turf-type tall fescue (Festuca arundinacea
Schreb.) germplasm available both commercially
and in cultivar trials is surprisingly similar
in quality and performance. To test genetic
diversity within this germplasm, Diversity Arrays
Technology (DArT) was used to detect DNA
polymorphism among 93 entries from the 2006
National Turfgrass Evaluation Program tall fescue
test in Riverside, CA. Based on the analysis
of 190 polymorphic DArT markers, we found
little variability in the turf-type cultivars. These
data are in agreement with fi eld observations
showing very few distinguishable differences in
turf quality among the entries, with the exception
of ‘Kentucky-31’, a forage-type standard
entry. This cultivar ranked lowest for turf quality.
The 93 entries were then compared with 14
forage-type and four turf-type accessions collected
worldwide, further demonstrating narrow
diversity among the entries and overall lower
genetic diversity among turf-types relative to
forage-types. Such low genetic polymorphism
of turf-type genotypes indicates a very close
relationship regardless of the origin. It may have
been caused by either a severe genetic bottleneck
in the conversion of germplasm from forage
to turfgrass use, or by extensive sharing of
germplasm. This indicates an urgent need to
rapidly broaden the genetic diversity in commercial
germplasm of turf-type tall fescue.
Fulltext: contact IEB authors
IEB authors: Jan Bartoš,
Jaroslav Doležel,
David Kopecky
Čeština
English