Mayer, K.F.X., Taudien, S., Martis, M., Šimková, H., Suchánková, P., Gundlach, H., Wicker, T., Petzold, A., Felder, M., Steuernagel, B., Scholz, U., Graner, A., Platzer, M., Doležel, J., Stein, N.
PLANT PHYSIOLOGY
151:
496-505,
2009
Klíčová slova:
Flow cytometry, barley, chromosomes, sequencing, rice, sorghum, genes, expressed sequence tag, Triticeae, synteny
Abstrakt:
Chromosome 1H (approximately 622 Mb) of barley (Hordeum vulgare) was isolated by flow sorting and shotgun sequenced by
GSFLX pyrosequencing to 1.3-fold coverage. Fluorescence in situ hybridization and stringent sequence comparison against
genetically mapped barley genes revealed 95% purity of the sorted chromosome 1H fraction. Sequence comparison against the
reference genomes of rice (Oryza sativa) and sorghum (Sorghum bicolor) and against wheat (Triticum aestivum) and barley
expressed sequence tag datasets led to the estimation of 4,600 to 5,800 genes on chromosome 1H, and 38,000 to 48,000 genes in
the whole barley genome. Conserved gene content between chromosome 1H and known syntenic regions of rice chromosomes
5 and 10, and of sorghum chromosomes 1 and 9 was detected on a per gene resolution. Informed by the syntenic relationships
between the two reference genomes, genic barley sequence reads were integrated and ordered to deduce a virtual gene map of
barley chromosome 1H. We demonstrate that synteny-based analysis of low-pass shotgun sequenced flow-sorted Triticeae
chromosomes can deliver linearly ordered high-resolution gene inventories of individual chromosomes, which complement
extensive Triticeae expressed sequence tag datasets. Thus, integration of genomic, transcriptomic, and synteny-derived
information represents a major step toward developing reference sequences of chromosomes and complete genomes of the
most important plant tribe for mankind.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Hana Šimková,
Pavla Suchánková
Čeština
English