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Laboratory of DNA repair and molecule farm   Field of research | Workers | Research projects | References | TAGIP

The laboratory is studying proceses underlying induction of DNA mutations, repair and recombination in plants under various stress conditions.
Because only a very few suitable mutants are available, the understanding of these processes in plants lag behind other organisms. To circumvent this obstacle our strategy takes the advantage of Agrobacterium tumefaciens mediated transformation: Firstly to overexpress exogenous yeast repair genes (Rad6 and Rad52) in tobacco to study their effects, secondly to produce and screen Arabidopsis T-DNA insertional library with enhancer sequence for sensitivity to chemical mutagens. We have developed the cotyledon assay for detection of induced mutagenesis in tobacco and the comet assay for detection of DNA damage in virtually any plant.
 
 
 
  Workers

Pascale Schlaich, Dita Bohdanecká,  Markéta Šmídková, Jitka Brouzdová, Jarda Kozák, Karel Angelis, Marcela Holá

	RNDr. Karel J. Angelis, CSc. head of the laboratory
Ing. Dita Bohdanecká protein expression in plants and purification
	Ing. Jitka Brouzdová protein expression in plants
Mgr. Marcela Holá production of bioactiv proteins in moss  Physcomitrella patents
	Mgr. Jaroslav Kozák DNA repair
Ing. Stanislava Kuviková, PhD. maternity leave
	Mgr. Markéta Šmídková protein production in moss  Physcomitrella patents, production scFv in plants

 

 
  Research projects

• The moss Physcomitrella patents as production crop, Grant Agency of the Czech Republic, 2004 - 2006
• Studies of DNA reparation in Arabidopsis thaliana, Grant Agency of the Czech Republic
• Target gene integration in plants: vectors, mechanisms and applications for protein production, 2005 - 2007
• Centre of targeted terapeutics, Ministry of Education, 2005 - 2009
  
• Integrated research of plant genome, Ministry of Education, 2006 - 2010
  

  Finished projects

Grant Agency of the Czech Republic:

• The role of DNA double-strand breaks on plant genome stability, 1996-1998
• Analysis of plant gene regulation systems by constitutive gene activation, 1997-1999
• Biochemical and genetic response of seeds to ionizing and microwave irradiation, 1998-2000
• Production of human papillomavirus proteins (PVP) in transgenic plants: an attempt to develop edible vaccines 2000-2002 - Abstract
• The role of plant systemic acquired resistance (SAR) in response to genotoxic stress 2001-2003 - Abstract

DFG:

• Einfluß der Expression von heterologen Proteinen auf DNA Reparatur und Genomstabilität im Tabak, 1997-1999
• Potenz und molekulare Grundlagen des Nachweises von Genomschädigungen über “single cell electrophoresis” (Comet-Assay) bei Pflanzen, 1998-2000

 
  References

Friedlender M, S Lev-Yagun, I Babůrek, K Angelis, AA Levy (1996) Cell divisions in cotyledons after germination: localization, time course and utilization for mutagenesis assay. Planta 199:307-313

Babůrek I,B Stibůrková, AA Levy, KJ Angelis (1997) Tobacco cotyledons: A Novel System for Testing Mutagenicity in Plants. Env. Molec. Mutag. 30:91-93

Koppen G, KJ Angelis (1998) Repair of X-ray induced DNA damage measured by comet assay in root tips of Vicia faba. Env. Molec. Mutag. 32:281-285

Angelis KJ, M Dušínská, AR Collins (1999) Single cell gel electrophoresis; detection of DNA damage at different levels of sensitivity. Electrophoresis 20: 2133-2138

Menke M, KJ Angelis, I Schubert (2000) Detection of specific DNA lesions by a combination of comet assay and FISH in plants. Env. Molec. Mutag. 35:132-138

Angelis KJ, M McGuffie, M. Menke, I Schubert (2000) Adaptation to Alkylation Damage in DNA Measured by the Comet assay. Env. Molec. Mutag. 36: 146-150

Menke M, I-P Cheng, KJ Angelis, I Schubert (2001) DNA damage and repair in Arabidopsis thaliana as measured by the Comet Assay after treatment with different classes of genotoxins. Mutat. Res. 493: 87-93