Department of Pharmacology
The production of nitric oxide by mouse peritoneal cells can be used as a biomarker predicting the cytokine-stimulatory effects of drugs in human cells.
Some acyclic nucleoside phosphonates (A0 – F1) are potent stimulators of chemokine MIP-1α in human cells (MIP-1α inhibits the entry of HIV-1 into cells).
Sesquiterpene lactone trilobolide, an inhibitor of Ca2+ATP-ase, activates the secretion of interferon-g (IFN-γ) in rat and human immunocompetent cells.
Current grant support
Ministry of Education,1M0508, Center for New Antivitals and Antineoplastics, 2005–2011.
GA CR, 305/07/0061, Immunopharmacological potential of endoplasmic Ca2+-ATPase SERCA, 2007–2011.
GA CR, 305/08/0535, Interference of probiotics with factors determining pharmacokinetics of drugs, 2008–2011.
Selected recent publications
1. Zídek Z, Potměšil P, Kmoníčková E, Holý A. (2003) Immunobiological activity of N-[2- (phosphonomethoxy)alkyl] derivatives of N6-substituted adenines, and 2,6-diamunopurines. Eur J Pharmacol 475: 149–159. PDF file (279 kB)
2. Kmoníčková E, Potměšil P, Holý A, Zídek Z. (2006) Purine P1 receptor-dependent mmunostimulatory effects of antiviral acyclic analogues of adenine and 2,6-diaminopurine. Eur J Pharmacol 530(1–2): 179–187. PDF file (795 kB)
3. Potměšil P, Holý A, Kmoníčková E, Křížková J, Zídek Z. (2007) Acyclic nucleoside hosphonate antivirals activate gene expression of monocyte chemotactic protein 1 and 3. J Biomed Sci 14: 59–66. PDF file (291 kB)
4. Zídek Z, Kmoníčková E, Holý A. (2007) Secretion of antiretroviral chemokines by human cells cultured with acyclic nucleoside phosphonates. Eur J Pharmacol 574: 77–84. PDF file (565 kB)
5. Kmoníčková E, Melkusová P, Farghali H, Holý A, Zídek Z. (2007) Nitric oxide production in mouse and rat macrophages: A rapid and efficient assay for screening of drugs immunostimulatory effects in human cells. Nitric Oxide 17: 160–169. PDF file (284 kB)
6. Kmoníčková E, Melkusová P, Harmatha J, Vokáč K, Farghali H, Zídek Z. (2008) Inhibitor of sarco-endoplasmic reticulum Ca2+-ATPase thapsigargin stimulates production of nitric oxide and secretion of interferon-gamma. Eur J Pharmacol 588(1): 85–92. PDF file (874 kB)
7. Zídek Z, Anzenbacher P, Kmoníčková E. (2009) Current status and challenges of cytokine pharmacology (Review). Br J Pharmacol 157: 342–361.
