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DNA Engine Opticon 2
| Location: |
Biochemical Pharmacology of Antimetabolites (Dr. Helena Mertlíková-Kaiserová) |
| Responsible person: |
Dr. Helena Mertlíková-Kaiserová (phone 220 183 114) |
The DNA Engine Opticon 2 (MJ Research) Real-Time PCR Detection System has two-color detection capability and a sensitive optical system with no
moving parts. Samples are illuminated by a fixed array of 96 blue-green LEDs and detected by two photomultiplier tubes (PMTs).
The first PMT detects at 523–543 nm (suitable for detecting SYBR Green I and FAM-labeled probes), while the second detects at 540–700 nm
(suitable for detecting many commonly used fluorophores, including HEX-, TET-, TAMRA-, and VIC dye-labeled probes).
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