Plchova H., Moravec T., Dedic P., Cerovska N.
JOURNAL OF PHYTOPATHOLOGY
159:
130-132,
2011
Klíčová slova:
Potato leafroll virus, recombinant viral antigen, antibody production
Abstrakt:
Vector pMPM-A4omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)–fragments RdRp43-616 and RdRp304-537
were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system
using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
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Autoři z ÚEB: Noemi Čeřovská,
Tomas Moravec,
Helena Plchová
Čeština
English