Kopecký, D., Bartoš, J., Lukaszewski, A.J., Baird, J.H., Černoch, V., Kölliker, R., Sandve, S.R., Rognli, O.A., Blois, H., Caig, V., Doležel, J., Kilian, A.
In: Huyghe, Ch. (ed.): Sustainable use of genetic diversity in forage and turf breeding. Springer.
:
443-448,
2010
Klíčová slova:
DArT, Fescue, Hybrids, Introgression, Mapping, Ryegrass
Abstrakt:
With the ďm to facilitate high-throughput genome profiling and genetic
and physical mapping within the Festuca-Inlium, gomplex, we have developed
a Diversity Arrays Technology (DArT) array for five important species: Festuca
pratensis, Festuca arundinacea, Festuca glaucescens, Inlium perenne and Lolium
multiflorum. The DArTFest array contains 7,680 probes derived from methylfiltered
genomic representations. Of 3,884 polymorphic DArT markers identified
in the first marker discovery experiment, over 1,000 markers detected a positive
allele in each species.
We assigned DArT markers to individuď chromosome regions of F. pratensis
using a series of single chromosome substitution and recombinant lines of E pratensis
in Z. multiflorum. Moreover, we enriched the existing genetic map of F. pratensis
by over 200 DArT markers and existing genetic map of Z. multiflorum by over 500
DAIT markers. The resonrces developed in this project will facilitate development
of genetic maps in Festuca and I'olium, the anďysis of genomic constitution in Festuca x Lolium hybrids, as well as marker-assisted selection for multiple traits.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Jaroslav Doležel,
David Kopecky
Čeština
English