Doležel, J., Šimková, H., Kubaláková, M., Šafář, J., Suchánková, P., Číhalíková, J., Bartoš, J., Valárik, M.
In: Feuillet, C., Muehlbauer, G.J. (eds.): Genetics and Genomics of the Triticeae. Springer, New York.
:
285-316,
2009
Klíčová slova:
Wheat, Triticeae, physical mapping, chromosomes, flow-sorting, BAC libraries
Abstrakt:
The Triticeae species are unique among the important agricultural
crops in possessing massive genomes with a prevalence of dispersed DNA
repeats. The highest level of complexity is observed in tetraploid and hexaploid
wheat whose nuclear genomes comprise two and three homoeologous genomes,
respectively. Polyploidy and the presence of repeats make gene cloning and
genome sequencing in the Triticeae extremely difficult. Chromosome genomics
simplifies these tasks by targeting single chromosomes and chromosome arms,
which represent only a few percent of the nuclear genomes. The advantages of
this strategy over a whole-genome approach include the avoidance of problems
due to the presence of homoeologs in wheat, reduction of work to manageable
portions, cost efficiency, and an opportunity to structure collaborative projects
where individual laboratories work on particular chromosomes. In this chapter,
we describe how chromosomes and chromosome arms can be isolated by flow
cytometric sorting and we review development of flow cytogenetics in the
Triticeae. We then discuss various applications of flow-sorted chromosomes
and assess the potential of chromosome genomics in the Triticeae.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Jarmila Číhalíková,
Jaroslav Doležel,
Marie Kubaláková,
Jan Šafář,
Hana Šimková,
Pavla Suchánková,
Miroslav Valárik
Čeština
English