Gichner, Tomáš; Lovecká, P.; Kochánková, L.; Macková, M.; Demnerová, K.
MUTATION RESEARCH - GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
629 [1]:
1-6,
2007
Klíčová slova:
comet assay; single-cell gel electrophoresis; gas chromatography
Abstrakt:
Heterozygous tobacco (Nicotiana tabacum var. xanthi) plants were cultivated in soil from a dump site highly polluted with polychlorinated biphenyls (PCBs) at Lhenice in South Bohemia, Czech Republic. The total amount of PCBs in the polluted soil, measured by gas chromatography varied from 165 to 265 mg kg(-1) of soil. In tobacco plants cultivated for 8 weeks in the polluted soil the amount of PCBs in the leaves varied from 11 to 28 and in the roots from 104 to 308 mg kg(-1) dry mass. The average leaf area of tobacco plants growing in the PCB-polluted soil was significantly reduced and the DNA damage in leaf nuclei, measured by the comet assay, was slightly but significantly increased compared with controls. The tobacco plants with increased DNA damage showed reduced growth and had distorted leaves. No increase in the frequency of somatic mutations was detected in tobacco plants growing in the PCB-polluted soil.
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