Berkman, P.J., Skarshewski, A., Lorenc, M.T., Lai, K., Duran, Ch., Ling, E.Y.S., Stiller, J., Smits, L., Imelfort, M., Manoli, S., McKenzie, M., Kubaláková, M., Šimková, H., Batley, J., Fleury, D., Doležel, J., Edwards, D.
PLANT BIOTECHNOLOGY JOURNAL
9:
768-775,
2011
Klíčová slova:
wheat genome sequence, chromosome 7, genome assembly, gene annotation
Abstrakt:
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in
size and consist predominantly of repetitive elements, making the sequencing and assembly
of this genome a major challenge. We have reduced genome sequence complexity by isolating
chromosome arm 7DS and applied second-generation technology and appropriate algorithmic
analysis to sequence and assemble low copy and genic regions of this chromosome
arm. The assembly represents approximately 40% of the chromosome arm and all known
7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza
sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic
relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping
data, has been used to produce an annotated draft 7DS syntenic build, which is publicly
available at http://www.wheatgenome.info. Our results suggest that the sequencing of isolated
chromosome arms can provide valuable information of the gene content of wheat and
is a step towards whole-genome sequencing and variation discovery in this important crop.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková
Čeština
English