TaaPmt4ALhA

LIBRARY DESCRIPTION
LIBRARY CODE TaaPmt4ALhA
SPECIES, CULTIVAR Triticum aestivum cv. reconstructed chromosome arm
SPECIFICITY 4AL
DATE OF CONSTRUCTION 07/2011
PLACE OF ORIGIN Olomouc, Czech Republic
AUTHORS Šimková, Šafář
CLONING SITE HindIII
NUMBER OF CLONES 43 008
NUMBER OF PLATES 112
NUMBER OF LIGATIONS 3
AVERAGE INSERT SIZE 113kb
EMPTY CLONES 10.0%
PURITY OF THE SORTED FRACTION 85%
GENOME/CHROMOSOME COVERAGE 6.8x
ANTIBIOTIC RESISTANCE Chloramphenicol (12.5 µg/ml)
HOST Escherichia coli MegaX DH10B T1
NOTES

 B1: plates no.: 0001 - 0051, (19,584 clones, av. insert-size:110kb, coverage: 3.4x)
  M: plates no.: 0052 - 0069, (6,912 clones, av. insert-size:141kb, coverage: 1.4x)
B2: plates no.: 0070 - 0112, (16,512 clones, av. insert-size:107kb, coverage: 2.0x)
 
Composite 4AL chromosome arm consists o fsegments of two cultivars of Triticum aestivum (Chinese Spring and Tahti) and from segment originating from T. militinae

AVAILABLE RESOURCES