Research topics

Modulation of microtubule properties by signal transduction molecules, function of gamma-tubulin forms, microtubule-associated proteins, interactions between microtubules and other cellular components. Tubulins as markers of tumor cells.

Current grant support

Ministry of Education, Youth and Sports of the Czech Republic (Center of Cell Functional Organization, grant No.: LC545)
Grant Agency of the Czech Republic (grants Nos.: 204/09/1777, 204/09/H084, P302/10/1701, P302/11/P709)
Grant Agency of the Academy of Sciences of the Czech Republic (KAN200520701)
Technology Agency of the Czech Republic (TA01010436)

Most important recent papers

(2006-present; names of the lab members underlined)

Katsetos CD, Reddy G, Draberova E, Smejkalova B, Del Valle L, Ashraf Q, Tadevosyan A, Yelin K, Maraziotis T, Mishra OP, Mork S, Legido A, Nissanov J, Baas PW, de Chadarevian JP, Draber P. Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines. J Neuropathol Exp Neurol 65(5): 465-77, 2006. (Pubmed) (DOI)

Sulimenko V, Draberova E, Sulimenko T, Macurek L, Richterova V, Draber P, Draber P. Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol 176(12): 7243-53, 2006. (Pubmed)

Peknicova J, Pexidrova M, Kubatova A, Koubek P, Tepla O, Sulimenko T, Draber P. Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil Steril 88(4 Suppl): 1120-8, 2007. (Pubmed) (DOI)

Katsetos CD, Draberova E, Smejkalova B, Reddy G, Bertrand L, de Chadarevian JP, Legido A, Nissanov J, Baas PW, Draber P. Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells. Neurochem Res 32(8): 1387-98, 2007. (Pubmed) (DOI)

Draberova E, Del Valle L, Gordon J, Markova V, Smejkalova B, Bertrand L, de Chadarevian JP, Agamanolis DP, Legido A, Khalili K, Draber P, Katsetos CD. Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity. J Neuropathol Exp Neurol 67(4): 341-54, 2008. (Pubmed) (DOI)

Blume Y, Yemets A, Sulimenko V, Sulimenko T, Chan J, Lloyd C, Draber P. Tyrosine phosphorylation of plant tubulin. Planta 229(1): 143-50, 2008. (Pubmed) (DOI)

Macurek L, Draberova E, Richterova V, Sulimenko V, Sulimenko T, Draberova L, Markova V, Draber P. Regulation of microtubule nucleation from membranes by complexes of membrane-bound gamma-tubulin with Fyn kinase and phosphoinositide 3-kinase. Biochem J 416(3): 421-30, 2008. (Pubmed) (DOI)

Draberova E, Sulimenko V, Sulimenko T, Bohm KJ, Draber P. Recovery of tubulin functions after freeze-drying in the presence of trehalose. Anal Biochem 397(1): 67-72, 2010. (Pubmed) (DOI)

Blume Y, Yemets A, Sheremet Y, Nyporko A, Sulimenko V, Sulimenko T, Draber P. Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol 10(1): 29, 2010. (Pubmed) (DOI)

Caracciolo V, D'agostino L, Draberova E, Sladkova V, Crozier-Fitzgerald C, Agamanolis DP, de Chadarevian JP, Legido A, Giordano A, Draber P, Katsetos CD. Differential expression and cellular distribution of gamma-tubulin and betaIII-tubulin in medulloblastomas and human medulloblastoma cell lines. J Cell Physiol 223(2): 519-29, 2010. (Pubmed) (DOI)

Hajkova Z, Bugajev V, Draberova E, Vinopal S, Draberova L, Janacek J, Draber P, Draber P. STIM1-directed reorganization of microtubules in activated mast cells. J Immunol 186(2): 913-23, 2011. (Pubmed) (DOI)

Horejsi B, Vinopal S, Sladkova V, Draberova E, Sulimenko V, Sulimenko T, Vosecka V, Philimonenko A, Hozak P, Katsetos CD, Draber P. Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol. 2011 Apr 4. doi: 10.1002/jcp.22772. [Epub ahead of print] (Pubmed) (DOI)

Dráberová E., Vinopal S., Morfini G., Liu P.S., Sulimenko T., Sládková V., Burns M.A., Solowska J., Kulandaivel K., de Chadarévian J.-P., Legido A., Mörk S.J., Janáček J., Baas P.W., Dráber P., Katsetos C.D. Microtubule-severing ATPase spastin in glioblastoma multiforme: increased expression in human glioblastoma cell lines and inverse roles in cell motility and proliferation. J. Neuropathol. Exp. Neurol. (in press)

Reviews

Binarova P, Cenklova V, Pochylova Z, Draberova E, Draber P. Gamma-tubulins and their functions in plant cells. In: The Plant Cytoskeleton. Eds: YB Blume, VW Baird, AI Yemets, D Breviario. Springer Verlag, ISBN: 987-1-4020-8841-4, pp 23-43, 2008.

Katsetos CD, Draberova E, Legido A, Dumontet C, Draber P. Tubulin targets in the pathobiology and therapy of glioblastoma multiforme. I. Class III beta-tubulin. J Cell Physiol 221(3): 505-13, 2009. (Pubmed) (DOI)

Katsetos CD, Draberova E, Legido A, Draber P. Tubulin targets in the pathobiology and therapy of glioblastoma multiforme. II. gamma-Tubulin. J Cell Physiol 221(3): 514-20, 2009. (Pubmed) (DOI)

Katsetos CD, Draber P, Kavallaris M. Targeting III-Tubulin in Glioblastoma Multiforme: From Cell Biology And Histopathology To Cancer Therapeutics. Anticancer Agents Med Chem. 2011 Jun 27. [Epub ahead of print] (Pubmed)

Other results

Monoclonal antibodies against cytoskeletal proteins (link) + (Exbio).

Teaching, lectures, public presentations