Synthesis of linear peptides

• Automatic solid-phase synthesizer ABI 433A (Applied Biosystems)
• Crude peptides are purified on preparative HPLC (Ingos, column C18)
• For characterized pure peptides we use analytical HPLC (Waters, Alliance)

Protein Sequencing

The chemical process used in the Procise system is derived from the technique developed by Pehr Edman in the 1950’s for the sequential degradation of proteins and peptides. In the Edman degradation, a protein’s N-terminal amino acid is specifically reacted with phenylisothiocyanate (PITC). This derivatized aminoacid is then selectively removed, leaving the rest of the peptide chain intact. Each cycle of the degradation removes the new N-terminal amino acid from the peptide chain. The resulting PTHamino acids are analyzed sequentially (by RP HPLC) to determine the amino acid sequence of the protein or peptide. The Team operates the Procise 491 HT Protein Sequencing System and the Procise 494 cLC Protein Sequencing System (high sensitivity analyses at sub-picomole level).

Amino Acid Analysis

Amino acid analysis is a technique based on ion exchange liquid chromatography, used in a wide range of application areas to provide qualitative and quantitative compositional analysis. The Biochrom 30 Amino Acid Analyser, is designed to provide accurate quantitative analysis of amino acid mixtures. The basic principle of operation is the continuous flow chromatography procedure developed by Spackman, Moore and Stein in 1958. In the Biochrom 30 this basic principle has been refined to produce fully automatic, high speed, sensitive analyses.