Muñoz-Amatriaín, M., Moscou, M.J., Bhat, P.R., Svensson, J.T., Bartoš, J., Suchánková, P., Šimková, H., Endo, T.R., Fenton, R.D., Lonardi, S., Castillo, A.M., Chao, S., Cistué, L., Cuesta-Marcos, A., Forrest, K.L., Hayden, M.J., Hayes, P.M., Horsley, R.D., Makoto, K., Moody, D., Sato, K., Vallés, M.P., Wulff, B.B.H., Muehlbauer, G.J., Doležel, J., Close, T.J.
THE PLANT GENOME
4 (3):
238-249,
2011
Klíčová slova:
Abstrakt:
Recent advances in high-throughput genotyping have made it
easier to combine information from different mapping populations
into consensus genetic maps, which provide increased marker
density and genome coverage compared to individual maps.
Previously, a single nucleotide polymorphism (SNP)-based
genotyping platform was developed and used to genotype
373 individuals in four barley (Hordeum vulgare L.) mapping
populations. This led to a 2943 SNP consensus genetic map
with 975 unique positions. In this work, we add data from six
additional populations and more individuals from one of the
original populations to develop an improved consensus map from
1133 individuals. A stringent and systematic analysis of each of
the 10 populations was performed to achieve uniformity. This
involved reexamination of the four populations included in the
previous map. As a consequence, we present a robust consensus
genetic map that contains 2994 SNP loci mapped to 1163
unique positions. The map spans 1137.3 cM with an average
density of one marker bin per 0.99 cM. A novel application
of the genotyping platform for gene detection allowed the
assignment of 2930 genes to flow-sorted chromosomes or
arms, confirmed the position of 2545 SNP-mapped loci, added
chromosome or arm allocations to an additional 370 SNP loci,
and delineated pericentromeric regions for chromosomes 2H
to 7H. Marker order has been improved and map resolution
has been increased by almost 20%. These increased precision
outcomes enable more optimized SNP selection for markerassisted
breeding and support association genetic analysis
and map-based cloning. It will also improve the anchoring of
DNA sequence scaffolds and the barley physical map to the
genetic map.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Jaroslav Doležel,
Hana Šimková,
Pavla Suchánková
Čeština
English