HomePh.D. Study at IOCBArchive - 2009Microbiology (2009)

Microbiology (2009)

Tutor Ing. Marie Zarevúcka, CSc.
Team Irena Valterová
Topic Enzymatic reactions in supercritical fluids
Faculty FPBT VŠCHT
Abstract

Enzymic reactions in supercritical carbon dioxide combine the advantage of biocatalysis (substrate specifity under mild reaction conditions) and supercritical fluids (high mass-transfer rate, easy separation of reaction products from the solvent, environmental benefits).

PhD student will study the following tasks:
i) isolation and preparation of enzymes from Geotrichum candidum
ii) testing the enzyme activity and stability in supercritical carbon dioxide
iii) investigating the enzyme reaction in a continuous-flow reactor and the conversion    of the reactions and specificity of enzymes,
iv) understanding the kinetic, thermodynamic, and transport phenomena and their influence on the enzyme-catalyzed reactions.