Dobrev, Petre; Havlíček, Libor; Vágner, Martin; Malbeck, Jiří; Kamínek, Miroslav
JOURNAL OF CHROMATOGRAPHY
1075:
159-166,
2005
Keywords:
Auxin; Abscisic acid; Solid phase extraction
Abstract:
A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in “heart cutting” mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
IEB authors: Petre I. Dobrev,
Libor Havlíček,
Miroslav Kamínek,
Jiří Malbeck,
Martin Vágner
Čeština
English