Tomaszewski, C., Byrne, S.L., Foito, A., Kildea, S., Kopecký, D., Doležel, J., Heslop-Harrison, J.S., Stewart, D., Barth, S.
PLANT BREEDING
131:
345-349,
2012
Keywords:
Lolium perenne, perennial ryegrass, genetic map, crown rust, Puccinia coronata, DArT
Abstract:
Perennial ryegrass is the principal forage grass species used in
temperate agriculture. In recent years, significant efforts have been
made to develop molecular marker strategies to allow cost-effective
characterization of a large number of loci simultaneously. One such
strategy involves using DArT markers, and a DArT array has
recently been developed for the Lolium-Festuca complex. In this
study, we report the first use of the DArTFest array to generate a
genetic linkage map based on 326 markers in a Lolium perenne F2
population, consisting of 325 genotypes. For proof of concept, the
map was used to identify QTL associated with differences in crown
rust susceptibility, caused by the fungal biotroph, Puccinia
coronata.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
David Kopecky