Milec Z.,Tomková L., Sumíková T., Pánková K.
MOLECULAR BREEDING
30:
317-323,
2012
Klíčová slova:
Wheat, Vrn-B1, PCR marker, Multiplex PCR
Abstrakt:
Winter wheat requires vernalization, a
long exposure to low but non-freezing temperatures,
to promote reproductive development. The vernali-zation requirement in bread wheat (Triticum aes-tivum L.) is mainly controlled by theVrn-1genes
that are located on chromosomes 5A, 5B and 5D.
Dominant alleles confer spring habit and are
epistatic to the recessive winter alleles which means
that spring varieties carry at least one dominant
allele. To date, two dominant and one recessiveVrn-B1alleles have been described. Vrn-B1a(formerly
designated asVrn-B1)differs from the wintervrn-B1allele by a large deletion in intron 1. Vrn-B1b
has an additional small deletion and is probably
derived from Vrn-B1a. The novel allele described
here and designated as Vrn-B1calso has a large
deletion within intron 1 but with different break-points fromVrn-B1aorb, and sequence duplication,
showing that this is an independently derived spring
allele. By combining an exon 1 primer with
previously published PCR primers it was possible
to develop a multiplex PCR that distinguished all four alleles simultaneously. The multiplex PCR was
validated by testing 320 winter wheat and 137
spring wheat varieties. This demonstrated that the
novel Vrn-B1c allele was present in 25 spring
varieties of diverse origin, showing this allele to be
widely distributed.
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