Doleželová, Marie; Valárik, Miroslav; Bartoš, Jan; Vrána, Jan; Číhalíková, Jarmila; Molnár-Láng, M.; Doležel, Jaroslav
GENOME
46:
893-905,
2003
Klíčová slova:
Chromosome sorting; fluorescence in situ hybridization; repetitive DNA sequences
Abstrakt:
Procedures for chromosome analysis and sorting using flow cytometry were developed for rye (Secale cereale L.). Chromosome 1R could be discriminated on a flow caryotype. Remaining rye chromosomes (2R-7R) could be discriminated and sorted from individual wheat-rye addition lines. Flow sorted chromosomes were identified by fluorescence in situ hybridization. PCR with chromosome-specific primers confirmed the identity of sorted fractions and indicated suitability of sorted chromosomes for physical mapping. The possibility to sort large numbers of chromosomes opens a way for the construction of large-insert chromosome-specific DNA libraries in rye.
Autoři z ÚEB: Jan Bartoš,
Jarmila Číhalíková,
Jaroslav Doležel,
Marie Doleželová,
Miroslav Valárik
Čeština
English