Home › Research

Intraradical dynamics of two coexisting isolates of the arbuscular mycorrhizal fungus Glomus intraradices sensu lato as estimated by real-time PCR of mitochondrial DNA

Karol Krak, Martina Janoušková, Petra Caklová, Miroslav Vosátka, Helena Štorchová
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 78: 3630-3637, 2012

Keywords: Coexistence, arbuscular mycorrhiza, real-time PCR, mitochondrial DNA
Abstract: Real-time PCR in nuclear ribosomal DNA (nrDNA) is becoming a well-established tool for the quantification of arbuscular mycorrhizal (AM) fungi, but this genomic region does not allow specific amplification of closely related genotypes. The large subunit of mitochondrial DNA (mtDNA) has a higher resolution power, but mtDNA-based quantification has not been previously explored in AM fungi. We applied real-time PCR assays targeting the large subunit of mtDNA to follow the development DNA dynamics of two isolates of Glomus intraradices s. l. coexisting in the roots of medic (Medicago sativa). The mtDNA-based quantification was compared to quantification in nrDNA.
Fulltext:
IEB authors: Helena Štorchová