Kopecký, D., Bartoš, J., Christelová, P., Černoch, V., Kilian, A., Doležel, J.
THEORETICAL AND APPLIED GENETICS
122:
355-363,
2011
Keywords:
Abstract:
Complementary attributes of Festuca and Lolium
grasses can be combined in hybrid cultivars called
Festuloliums, which are becoming increasingly popular
fodder crops and amenity plants. Genomic constitution of
commercially available Festuloliums was reported to vary
from almost equal representation of parental genomes to
apparent lack of one of them based on molecular cytogenetic
analyses and screening with a small set of DNA
markers, both approaches with limited resolution. Here, we
describe the use of the DArTFest array comprising 3,884
polymorphic DArT markers for characterization of genomes
in five Festulolium cultivars. In any of the cultivars,
the minimum number of informative markers, which discriminated
the parental Lolium and Festuca genomes was
361 and 171, respectively. Using the DArTFest array, it
was possible to determine hybrid genome constitution at
resolution which has never been achieved before and the
analysis of a set of randomly selected plants from each
cultivar provided information on genetic structure of outcrossing
Festulolium cultivars. In addition to a core set of
markers typical for each hybrid cultivar, markers occurring
at low frequency among the plants within each cultivar
were identified. Biological significance of genomic loci
associated with the rare markers is yet to be determined.
Finally, with the aim to simplify the use of DArTFest
arrays to characterize Festuca 9 Lolium hybrids, various
bulking strategies were compared. While all bulks were
suitable for identification of hybrids, only bulks of few
plants have been found to reveal the rare markers.
Fulltext: contact IEB authors
IEB authors: Jan Bartoš,
Pavla Christelová,
Jaroslav Doležel,
David Kopecky
Čeština
English