Bartoš, J., Sandve, S.R., Kölliker, R. Kopecký, D., Christelová, P., Stočes, Š., Østrem, L., Larsen, A., Kilian, A., Rognli, O-A, Doležel, J.
THEORETICAL AND APPLIED GENETICS
122:
1133-1147,
2011
Klíčová slova:
Abstrakt:
Species belonging to the Festuca–Lolium
complex are important forage and turf species and as such,
have been studied intensively. However, their out-crossing
nature and limited availability of molecular markers make
genetic studies difficult. Here, we report on saturation of
F. pratensis and L. multiflorum genetic maps using Diversity
Array Technology (DArT) markers and the DArTFest
array.The 530 and 149 DArT markers were placed on
genetic maps of L. multiflorum and F. pratensis, respectively,
with overlap of 20 markers, which mapped in both
species. The markers were sequenced and comparative
sequence analysis was performed between L. multiflorum,
rice and Brachypodium. The utility of the DArTFest array
was then tested on a Festulolium population FuRs0357 in an
integrated analysis using the DArT marker map positions to
study associations between markers and freezing tolerance.
Ninety six markers were significantly associated with
freezing tolerance and five of these markers were genetically
mapped to chromosomes 2, 4 and 7. Three genomic
loci associated with freezing tolerance in the FuRs0357
population co-localized with chromosome segments and
QTLs previously indentified to be associated with freezing
tolerance. The present work clearly confirms the potential
of the DArTFest array in genetic studies of the Festuca–
Lolium complex. The annotated DArTFest array resources
could accelerate further studies and improvement of desired
traits in Festuca–Lolium species.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Pavla Christelová,
Jaroslav Doležel,
David Kopecky
Čeština
English