Kopecký, D., Bartoš, J., Lukaszewski, A.J., Baird, J.H., Černoch, V., Kölliker, R., Rognli, O.A., Blois, H., Caig, V., Lübberstedt, T., Studer, B., Shaw, P., Doležel, J., Kilian, A.
BMC GENOMICS
10:
473,
2009
Klíčová slova:
Festuca, Lolium, DArT markers, genetic mapping
Abstrakt:
Background: Grasses are among the most important and widely cultivated plants on Earth. They provide
high quality fodder for livestock, are used for turf and amenity purposes, and play a fundamental role in
environment protection. Among cultivated grasses, species within the Festuca-Lolium complex
predominate, especially in temperate regions. To facilitate high-throughput genome profiling and genetic
mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five grass
species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum.
Results: The DArTFest array contains 7680 probes derived from methyl-filtered genomic
representations. In a first marker discovery experiment performed on 40 genotypes from each species
(with the exception of F. glaucescens for which only 7 genotypes were used), we identified 3884
polymorphic markers. The number of DArT markers identified in every single genotype varied from 821
to 1852. To test the usefulness of DArTFest array for physical mapping, DArT markers were assigned to
each of the seven chromosomes of F. pratensis using single chromosome substitution lines while
recombinants of F. pratensis chromosome 3 were used to allocate the markers to seven chromosome bins.
Conclusion: The resources developed in this project will facilitate the development of genetic maps in
Festuca and Lolium, the analysis on genetic diversity, and the monitoring of the genomic constitution of the
Festuca × Lolium hybrids. They will also enable marker-assisted selection for multiple traits or for specific
genome regions.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Jaroslav Doležel,
David Kopecky
Čeština
English