Molnár, I., Kubaláková, M., Šimková, H., Cseh, A., Molnár-Láng, M., Doležel, J.
PLoS ONE
6 (11):
e27708,
2011
Klíčová slova:
Abstrakt:
This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops
umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes
obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was
determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and
the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and
could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of
Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-
Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted
chromosomes, thus confirming the usefulness of this approach for physical mapping. The SSR markers are suitable for
marker assisted selection of wheat-Aegilops introgression lines. The results obtained in this work provide new opportunities
for dissecting genomes of wild relatives of wheat with the aim to assist in alien gene transfer and discovery of novel genes
for wheat improvement.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková
Čeština
English