Malá J., Gaudinová A., Dobrev P., Eder J., Cvikrová M.
BIOLOGIA PLANTARUM
5:
8-14,
2006
Klíčová slova:
auxin, cytokinins, multiplication of elm, phenolic substances, polyamines.
Abstrakt:
The study presents the comparative analyses of endogenous contents of auxin (IAA), cytokinins (CKs), polyamines
(PAs), and phenolic acids (PhAs) in apical and basal parts of elm multiplicated shoots with regard to the organogenic
potential. The shoot-forming capacity was higher in the apical part than in the basal part. However, the timing of root
formation was in the apical type of explant significantly delayed (compared with the organogenic potential of basal
part). Significantly higher contents of free bases, ribosides and ribotides of isopentenyl adenine, zeatin and dihydrozeatin
that were found in the apical segments, might be considered as the most important factor affecting in vitro shoot
formation. The content of endogenous free IAA was approximately three times higher in the basal shoot parts than in the
apical parts. The amounts of putrescine and spermidine were higher in the apical part which generally contains less
differentiated tissues than the basal part of shoot. The predominant PhA in both types of explants was caffeic acid, and
concentrations of other PhAs decreased in the following order: p-coumaric, ferulic, sinapic, vanillic, chlorogenic,
p-hydroxybenzoic and gallic acids. The contents of all determined PhAs in their free forms and higher contents of
glycoside-bound p-coumaric, ferulic and sinapic acids, precursors for lignin biosynthesis, were found in the basal parts.
Autoři z ÚEB: Milena Cvikrová,
Petre I. Dobrev
Čeština
English