Nie, X., Li, B., Wang, L., Liu, P., Biradar, S.S., Li, T., Doležel, J., Edwards, D., Luo, M., Weining, S.
AMERICAN JOURNAL OF BOTANY
:
e369–e371,
2012
Keywords:
chromosome-arm-specific DNA; flow-sorted chromosomes; next-generation sequencing; Poaceae; Triticum aestivum ; wheat.
Abstract:
• Premise of the study: The aim of this study was to assess the feasibility of developing chromosome-arm-specifi c microsatellite
markers in wheat on a large scale based on chromosome survey sequences obtained with next-generation sequencing (NGS)
technology.
• Methods and Results: The Illumina Hi Seq2000 sequencing platform was used to sequence DNA of isolated wheat chromosome-
arm 7DL. The data were assembled and microsatellite loci were identifi ed computationally. In total, 16 315 microsatellites
were identifi ed from 161 061 assembled contigs. Thirty-three markers were randomly selected for validation across 20
diverse wheat cultivars. Two nulli-tetrasomic stocks were also screened to validate the specifi city of the newly developed
markers.
• Conclusions: This is the fi rst study on identifi cation of chromosome-arm-specifi c microsatellite markers using NGS technology.
These new chromosome-arm-specifi c markers will facilitate saturation of the 7DL genetic map, and their availability will
support genetic mapping and positional cloning in wheat.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel
Čeština
English