Luo, M.-C., Gu, Y.Q., You, F.M., Deal, K.R., Ma, Y., Hu, Y., Huo, N., Wang, Y., Wang, J., Chen, S., Jorgensen, C.M., Zhang, Y., McGuire, P.E., Pasternak, S., Stein, J.C., Ware, D., Kramer, M., McCombie, W.R., Kianian, S.F., Martis, M.M., Mayer, K.F.X., Sehgal, S.K., Li, W., Gill, B.S., Bevan, M.W., Šimková, H., Doležel, J., Weining, S., Lazo, G.R., Anderson, O.D., Dvorak, J.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
110:
7940-7945,
2013
Klíčová slova:
single nucleotide polymorphism, synteny, gene density, Oryza, BAC contig coassembly
Abstrakt:
The current limitations in genome sequencing technology require
the construction of physical maps for high-quality draft sequences
of large plant genomes, such as that of Aegilops tauschii, the wheat
D-genome progenitor. To construct a physical map of the Ae. tauschii
genome, we fingerprinted 461,706 bacterial artificial chromosome
clones, assembled contigs, designed a 10K Ae. tauschii
Infinium SNP array, constructed a 7,185-marker genetic map, and
anchored on the map contigs totaling 4.03 Gb. Using whole genome
shotgun reads, we extended the SNP marker sequences
and found 17,093 genes and gene fragments. We showed that
collinearity of the Ae. tauschii genes with Brachypodium distachyon,
rice, and sorghum decreased with phylogenetic distance
and that structural genome evolution rates have been high across
all investigated lineages in subfamily Pooideae, including that of
Brachypodieae. We obtained additional information about the evolution
of the seven Triticeae chromosomes from 12 ancestral chromosomes
and uncovered a pattern of centromere inactivation
accompanying nested chromosome insertions in grasses.We showed
that the density of noncollinear genes along the Ae. tauschii chromosomes
positively correlates with recombination rates, suggested
a cause, and showed that new genes, exemplified by disease resistance
genes, are preferentially located in high-recombination
chromosome regions.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Hana Šimková
Čeština
English