Berkman, P.J., Skarshewski, A., Manoli, S., Lorenc, M.T., Stiller, J., Smits, L., Lai, K., Campbell, E., Kubaláková, M., Šimková, H., Batley, J., Doležel, J., Hernandez, P., Edwards, D.
THEORETICAL AND APPLIED GENETICS
124:
423–432,
2012
Keywords:
Abstract:
Complex Triticeae genomes pose a challenge
to genome sequencing efforts due to their size and repetitive
nature. Genome sequencing can reveal details of
conservation and rearrangements between related genomes.
We have applied Illumina second generation sequencing
technology to sequence and assemble the low copy and
unique regions of Triticum aestivum chromosome arm
7BS, followed by the construction of a syntenic build based
on gene order in Brachypodium. We have delimited the
position of a previously reported translocation between
7BS and 4AL with a resolution of one or a few genes and
report approximately 13% genes from 7BS having been
translocated to 4AL. An additional 13 genes are found on
7BS which appear to have originated from 4AL. The gene
content of the 7DS and 7BS syntenic builds indicate a total
of *77,000 genes in wheat. Within wheat syntenic
regions, 7BS and 7DS share 740 genes and a common gene
conservation rate of *39% of the genes from the corresponding
regions in Brachypodium, as well as a common
rate of colinearity with Brachypodium of *60%. Comparison
of wheat homoeologues revealed *84% of genes
previously identified in 7DS have a homoeologue on 7BS
or 4AL. The conservation rates we have identified among
wheat homoeologues and with Brachypodium provide a
benchmark of homoeologous gene conservation levels for
future comparative genomic analysis. The syntenic build of
7BS is publicly available at http://www.wheatgenome.info.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková
