Hernandez, P., Martis, M., Dorado, G., Pfeifer, M., Gálvez, S., Schaaf, S., Jouve, N., Šimková, H., Valárik, M., Doležel, J., Mayer, K.F.X.
PLANT JOURNAL
69:
377–386,
2012
Klíčová slova:
wheat genome, chromosome sorting, genome zipper, grass comparative genomics, wheat shotgun chromosome, Triticeae genome
Abstrakt:
Wheat is the third most important crop for human nutrition in the world. The availability of high-resolution
genetic and physical maps and ultimately a complete genome sequence holds great promise for breeding
improved varieties to cope with increasing food demand under the conditions of changing global climate.
However, the large size of the bread wheat (Triticum aestivum) genome (approximately 17 Gb/1C) and the
triplication of genic sequence resulting from its hexaploid status have impeded genome sequencing of this
important crop species. Here we describe the use of mitotic chromosome flow sorting to separately purify and
then shotgun-sequence a pair of telocentric chromosomes that together form chromosome 4A (856 Mb/1C) of
wheat. The isolation of this much reduced template and the consequent avoidance of the problem of sequence
duplication, in conjunction with synteny-based comparisons with other grass genomes, have facilitated
construction of an ordered gene map of chromosome 4A, embracing ‡85% of its total gene content, and have
enabled precise localization of the various translocation and inversion breakpoints on chromosome 4A that
differentiate it from its progenitor chromosome in the A genome diploid donor. The gene map of
chromosome 4A, together with the emerging sequences of homoeologous wheat chromosome groups 4, 5
and 7, represent unique resources that will allow us to obtain new insights into the evolutionary dynamics
between homoeologous chromosomes and syntenic chromosomal regions.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Hana Šimková,
Miroslav Valárik
Čeština
English