Lucas, S.J., Akpınar, B.A., Kantar, M., Weinstein, Z., Aydınoglu, F., Šafář, J., Šimková, H., Frenkel, Z., Korol, A., Magni, F., Cattonaro, F., Vautrin, S., Bellec, A., Berges, H., Doležel, J., Budak, H.
PLoS ONE
8:
e59542,
2013
Klíčová slova:
Abstrakt:
Background: Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide and its production faces
pressing challenges, the solution of which demands genome information. However, the large, highly repetitive hexaploid
wheat genome has been considered intractable to standard sequencing approaches. Therefore the International Wheat
Genome Sequencing Consortium (IWGSC) proposes to map and sequence the genome on a chromosome-by-chromosome
basis.
Methodology/Principal Findings: We have constructed a physical map of the long arm of bread wheat chromosome 1A
using chromosome-specific BAC libraries by High Information Content Fingerprinting (HICF). Two alternative methods (FPC
and LTC) were used to assemble the fingerprints into a high-resolution physical map of the chromosome arm. A total of 365
molecular markers were added to the map, in addition to 1122 putative unique transcripts that were identified by
microarray hybridization. The final map consists of 1180 FPC-based or 583 LTC-based contigs.
Conclusions/Significance: The physical map presented here marks an important step forward in mapping of hexaploid
bread wheat. The map is orders of magnitude more detailed than previously available maps of this chromosome, and the
assignment of over a thousand putative expressed gene sequences to specific map locations will greatly assist future
functional studies. This map will be an essential tool for future sequencing of and positional cloning within chromosome 1A.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Jan Šafář,
Hana Šimková
Čeština
English