PROJECT DETAILS
| Name: |
|
Noninvasive physical monitoring of molecular interactions in regulation of gene expression |
| Principal investigator: |
|
Prof. Josef Štěpánek, Ph.D. |
| From: |
|
2005-01-01 |
|
| To: |
|
2007-12-31 |
Advanced optical methods capable to diagnose sensitively biomolecular
interactions in small quantities without the interaction site labeling,
will be developed and applied to study of nucleic acid – protein
interactions, in particular those playing important role in regulation
of gene expression and/or in „antisense“ therapy. Formation, structure
and biochemical activity of enzyme – nucleic acid complexes in respect
to the base sequence and/or nucleotide modification will be studied on
selected cleavage and regulation enzymes. Methodological developments
will comprise (i) technical and mathematical solution for precise Raman
spectroscopy in nmol quantities, (ii) construction of high-resolution
surface plasmon resonance sensor systém with up to eight real-time
observation channels, (iii) design of complex biomolecular interaction
models considering effect of hydrodynamic conditions, (iv) preparation
of structurally diverse sugar – phosphate backbone modified
oligodeoxynucleotides.
