| Abstract |
Using nanoparticles for tumor targeting is a promising way for cancer therapy and diagnostics. Contrary to molecules and microparticles, nanoparticles posses number of usefull properties. They have ideal size which allows penetration and interaction with cell surface. Further, functional architectures on nanoparticles surface can be bound in polyvalent arrangement (large number of interacting molecules on a small surface area enhances interaction with cell receptors) and it is also possible to employ physical phenomena common for nanoparticles (plasmon rezonance). Combination of previously mentioned properties opens up the possibility of theranostics – integration of therapeutic and diagnostic functions into one type of particle.
Ph.D. thesis will be focused on preparation, optimization and application of new theranostic nanoparticles. Following topics will be investigated:
• Viral capsid protein (nanobiotechnological building blocks) expression
• Preparation of monodisperse biocompatible nanoparticles from viral capsids and surface functionalization of nanoparticles by bioorthogonal reactive groups
• Preparation of hybrid bioorganic-inorganic structures containing extremely stable fluorescent nanodiamonds
• Technique for formation of metalic layers with plasmonic behaviour on those particles
• Attachement of polyvalent architectures to nanoparticles surface and installation of targeting protein and peptidic structures
• Optimization of particles for theranostic aplications (combination of magnetic resonance imaging, optic and photoacoustic imaging, selective tumor destruction by using externally controlled pulses: laser ablation of tumors and drug release by pulse lasers).
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