Hoelzl C., Glatt H., Meinl M., Sontag G., Haidinger G., Kundi M., Simic T., Chakraborty A., Bichler J., Ferk F., Angelis K.J., Nersesyan A., Knasmüller S.
MOLECULAR NUTRITION & FOOD RESEARCH
52:
330-341,
2008
Klíčová slova:
Antioxidant, Comet assay, Cruciferous vegetables, Heterocyclic aromatic amines, Sulfotransferase
Abstrakt:
To find out if the cancer protective effects of Brussels sprouts seen in epidemiological studies are due
to protection against DNA-damage, an intervention trial was conducted in which the impact of vegetable
consumption on DNA-stability was monitored in lymphocytes with the comet assay. After consumption
of the sprouts (300 g/p/d, n = 8), a reduction of DNA-migration (97%) induced by the
heterocyclic aromatic amine 2-amino-1-methyl-6-phenyl-imidazo-[4,5-b]pyridine (PhIP) was
observed whereas no effect was seen with 3-amino-1-methyl-5H-pyrido[4,3-b]-indole (Trp-P-2). This
effect protection may be due to inhibition of sulfotransferase 1A1, which plays a key role in the activation
of PhIP. In addition, a decrease of the endogenous formation of oxidized bases was observed
and DNA-damage caused by hydrogen peroxide was significantly (39%) lower after the intervention.
These effects could not be explained by induction of antioxidant enzymes glutathione peroxidase and
superoxide dismutase, but in vitro experiments indicate that sprouts contain compounds, which act as
direct scavengers of reactive oxygen species. Serum vitamin C levels were increased by 37% after
sprout consumption but no correlations were seen between prevention of DNA-damage and individual
alterations of the vitamin levels. Our study shows for the first time that sprout consumption leads to
inhibition of sulfotransferases in humans and to protection against PhIP and oxidative DNA-damage.
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