Rustenholz, C., Choulet, F., Laugier, C., Šafář, J., Šimková, H., Doležel, J., Magni, F., Scalabrin, S., Cattonaro, F., Vautrin, S., Bellec, A., Bergès, H., Feuillet, C., Paux, E.
PLANT PHYSIOLOGY
157 (4):
1596-1608,
2011
Keywords:
Abstract:
To improve our understanding of the organization and regulation of the wheat (Triticum aestivum) gene space, we established a
transcription map of a wheat chromosome (3B) by hybridizing a newly developed wheat expression microarray with bacterial
artificial chromosome pools from a new version of the 3B physical map as well as with cDNA probes derived from 15 RNA
samples. Mapping data for almost 3,000 genes showed that the gene space spans the whole chromosome 3B with a 2-fold
increase of gene density toward the telomeres due to an increase in the number of genes in islands. Comparative analyses with
rice (Oryza sativa) and Brachypodium distachyon revealed that these gene islands are composed mainly of genes likely originating
from interchromosomal gene duplications. Gene Ontology and expression profile analyses for the 3,000 genes located along the
chromosome revealed that the gene islands are enriched significantly in genes sharing the same function or expression profile,
thereby suggesting that genes in islands acquired shared regulation during evolution. Only a small fraction of these clusters of
cofunctional and coexpressed genes was conserved with rice and B. distachyon, indicating a recent origin. Finally, genes with
the same expression profiles in remote islands (coregulation islands) were identified suggesting long-distance regulation of
gene expression along the chromosomes in wheat.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Jan Šafář,
Hana Šimková
Čeština
English