Jakobson, I., Reis, D., Tiidema, A., Peusha, H., Timofejeva, L., Valárik, M., Kladivová, M., Šimková, H., Doležel, J., Järve, K.
THEORETICAL AND APPLIED GENETICS
125:
609-623,
2012
Klíčová slova:
Abstrakt:
Introgression of several genomic loci from
tetraploid Triticum militinae into bread wheat cv. Ta¨hti has
increased resistance of introgression line 8.1 to powdery
mildew in seedlings and adult plants. In our previous work,
only a major quantitative trait locus (QTL) on chromosome
4AL of the line 8.1 contributed significantly to resistance,
whereas QTL on chromosomes 1A, 1B, 2A, 5A and 5B
were detected merely on a suggestive level. To verify and
characterize all QTLs in the line 8.1, a mapping population
of double haploid lines was established. Testing for seedling
resistance to 16 different races/mixtures of Blumeria
graminis f. sp. tritici revealed four highly significant nonrace-
specific resistance QTL including the main QTL on
chromosome 4AL, and a race-specific QTL on chromosome
5B. The major QTL on chromosome 4AL (QPm.tut-
4A) as well as QTL on chromosome 5AL and a newly
detected QTL on 7AL were highly effective at the adult
stage. The QPm.tut-4A QTL accounts on average for
33–49 % of the variation in resistance in the double haploid
population. Interactions between the main QTL
QPm.tut-4A and the minor QTL were evaluated and discussed.
A population of 98 F2 plants from a cross of susceptible
cv. Chinese Spring and the line 8.1 was created
that allowed mapping the QPm.tut-4A locus to the proximal
2.5-cM region of the introgressed segment on chromosome
4AL. The results obtained in this work make it feasible to
use QPm.tut-4A in resistance breeding and provide a solid
basis for positional cloning of the major QTL.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Monika Kladivová,
Hana Šimková,
Miroslav Valárik
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