Faricelli, M.E., Valárik, M., Dubcovsky, J.
Functional & Integrative Genomics
10:
293-306,
2010
Klíčová slova:
Comparative genomics, Earliness per se, Flowering, Wheat, Brachypodium
Abstrakt:
The earliness per se gene Eps-Am1 from diploid
wheat Triticum monococcum affects heading time, spike
development, and spikelet number. In this study, the Eps1
orthologous regions from rice, Aegilops tauschii, and
Brachypodium distachyon were compared as part of current
efforts to clone this gene. A single Brachypodium BAC
clone spanned the Eps-Am1 region, but a gap was detected
in the A. tauschii physical map. Sequencing of the
Brachypodium and A. tauschii BAC clones revealed three
genes shared by the three species, which showed higher
identity between wheat and Brachypodium than between
them and rice. However, most of the structural changes
were detected in the wheat lineage. These included an
inversion encompassing the wg241-VatpC region and the
presence of six unique genes. In contrast, only one unique
gene (and one pseudogene) was found in Brachypodium
and none in rice. Three genes were present in both
Brachypodium and wheat but were absent in rice. Two of
these genes, Mot1 and FtsH4, were completely linked to the
earliness per se phenotype in the T. monococcum highdensity
genetic map and are candidates for Eps-Am1. Both
genes were expressed in apices and developing spikes, as
expected for Eps-Am1 candidates. The predicted MOT1
protein showed amino acid differences between the parental
T. monococcum lines, but its effect is difficult to predict.
Future steps to clone the Eps-Am1 gene include the
generation of mot1 and ftsh4 mutants and the completion
of the T. monococcum physical map to test for the presence
of additional candidate genes.
Autoři z ÚEB: Miroslav Valárik
Čeština
English