Randhawa, M., Bansal, U., Valárik, M., Klocová, B., Doležel, J., Bariana, H.
THEORETICAL AND APPLIED GENETICS
127:
317-324,
2014
Klíčová slova:
Abstrakt:
Key message This manuscript describes the chromosomal
location of a new source of stripe rust resistance
in wheat. DNA markers closely linked with the resistance
locus were identified and validated.
Abstract A wheat landrace, AUS27858, from the Watkins
collection showed high levels of resistance against Australian
pathotypes of Puccinia striiformis f. sp. tritici. It was
reported to carry two genes for stripe rust resistance, tentatively
named YrAW1 and YrAW2. One hundred seeds of
an F3 line (HSB#5515; YrAW1yrAW1) that showed monogenic
segregation for stripe rust response were sown and
harvested individually to generate monogenically segregating
population (MSP) #5515. Stripe rust response variation
in MSP#5515 conformed to segregation at a single locus.
Bulked segregant analysis using high-throughput DArT
markers placed YrAW1 in chromosome 4AL. MSP#5515
was advanced to F6 and phenotyped for detailed mapping.
Novel wheat genomic resources including chromosomespecific
sequence and genome zipper were employed to
develop markers specific for the long arm of chromosome
4A. These markers were used for further saturation of the
YrAW1 carrying region. YrAW1 was delimited by 3.7 cMbetween markers owm45F3R3 and sun104. Since there
was no other stripe rust resistance gene located in chromosome
4AL, YrAW1 was formally named Yr51. Reference
stock for Yr51 was lodged at the Australian Winter Cereal
Collection, Tamworth, Australia and it was accessioned as
AUS91456. Marker sun104 was genotyped on a set of Australian
and Indian wheat cultivars and was shown to lack
the resistance-linked sun104-225 bp allele. Marker sun104
is currently being used for marker-assisted backcrossing of
Yr51 in Australian and Indian wheat backgrounds.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Barbora Klocová,
Miroslav Valárik
Čeština
English