Swaczynová, Jana; Novák, Ondřej; Hauserová, Eva; Fuksová, Květoslava; Šíša, Miroslav; Kohout, Ladislav; Strnad, Miroslav
JOURNAL OF PLANT GROWTH REGULATION
26 [1]:
1-14,
2007
Klíčová slova:
antibodies; brassinosteroids; enzyme immunoassay; ELISA
Abstrakt:
We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogs, with detection ranges of 0.005 to 50pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids.
Autoři z ÚEB: Ondřej Novák,
Miroslav Strnad