Feraru E., Vosolsobě S., Feraru I. M., Petrášek J., Kleine-Vehn J.
FRONTIERS IN PLANT SCIENCE
3:
,
2012
Klíčová slova:
PILS, auxin, evolution
Abstrakt:
The phytohormone auxin contributes to virtually every aspect of the plant development.
The spatiotemporal distribution of auxin depends on a complex interplay between auxin
metabolism and intercellular auxin transport. Intracellular auxin compartmentalization provides
another link between auxin transport processes and auxin metabolism.The PIN-LIKES
(PILS) putative auxin carriers localize to the endoplasmic reticulum (ER) and contribute to
cellular auxin homeostasis. PILS proteins regulate intracellular auxin accumulation, the rate
of auxin conjugation and, subsequently, affect nuclear auxin signaling. Here,we investigate
sequence diversification of the PILS family in Arabidopsis thaliana and provide insights into
the evolution of these novel putative auxin carriers in plants. Our data suggest that PILS
proteins are conserved throughout the plant lineage and expanded during higher plant
evolution. PILS proteins diversified early during plant evolution into three clades. Besides
the ancient Clade I encompassing non-land plant species, PILS proteins evolved into two
clades. The diversification of Clade II and Clade III occurred already at the level of nonvascular
plant evolution and, hence, both clades contain vascular and non-vascular plant
species. Nevertheless, Clade III contains fewer non- and increased numbers of vascular
plants, indicating higher importance of Clade III for vascular plant evolution. Notably, PILS
proteins are distinct and appear evolutionarily older than the prominent PIN-FORMED auxin
carriers. Moreover, we revealed particular PILS sequence divergence in Arabidopsis and
assume that these alterations could contribute to distinct gene regulations and protein
functions.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Petrášek
Čeština
English