Vrána, J., Šimková, H., Kubaláková, M., Číhalíková, J., Doležel, J.
METHODS
57:
331-337,
2012
Keywords:
Chromosome sorting, Flow cytometry, Fluorescence in situ hybridization, Next generation sequencing, Whole genome amplification
Abstract:
Genome analysis in many plant species is hampered by large genome size and by sequence redundancy
due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by
dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which
enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology
with next generation sequencing provides a targeted and cost effective way to tackle complex genomes.
The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable
for next-generation sequencing.
Fulltext: contact IEB authors
IEB authors: Jarmila Číhalíková,
Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková,
Jan Vrána
Čeština
English