Deng, P., Nie, X., Wang, L., Cui, L., Liu, P., Tong, W., Biradar, S.S., Edwards, D., Berkman, P., Šimková, H., Doležel, J., Luo, M., You, F., Batley, J., Fleury, D., Appels, R., Weining, S.
PLANT MOLECULAR BIOLOGY REPORTER
32:
487-500,
2014
Klíčová slova:
Wheat, miRNA, Group 7 chromosomes, Next-generation sequencing, Comparative genomics
Abstrakt:
MicroRNAs (miRNAs) are a class of important
biological elements that regulates gene expression by translational
repression or transcript degradation both in plants and
animals. The origin, distribution and evolution of the miRNA
genes have been systematically studied in many model plants,
but they are not well understood in wheat. In this study, we
have systematically identified the miRNA precursors and
mature miRNAs in wheat group 7 chromosomes by computational
analysis in the isolated chromosome sequences. A
total of 716 precursors belonging to 43 miRNA families were
identified, of which 12 miRNAs families (27.91 %) were not
reported in wheat. Further analysis found that there were less
mature miRNAs but more miRNA precursors distributing on
7D compared to 7A and 7B, suggesting that wheat group 7
homoeologous chromosome eliminated some miRNA precursors
with the potential to yield the same miRNA and formed a
more precise regulatory network via the two hybridization
events. The target genes of all the newly identified miRNAs
were also predicted. The results of this study are beneficial to
improve our understanding of the distribution of wheat
miRNAs and its evolutionary role in polyploidisation.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Hana Šimková
Čeština
English