Krumova S., Motyka V., Dobrev P., Todorova M., Trendafilova A., Evstatieva L., Danova K.
BULGARIAN JOURNAL of AGRICULTURAL SCIENCE
19:
26-30,
2013
Keywords:
Artemisia alba, in vitro, endogenous cytokinins, Photosystem II, plant growth regulators, terpenoid profile
Abstract:
Modifications of plant growth regulators supplied to Artemisia alba in vitro cultures have been previously shown to affect
plant morphogenesis and terpenoid profile of the essential oil of aerial parts. We report here on the effects of plant growth regulators
on the terpenoid profile, structure of photosystem II in vitro as well as on the endogenous cytokinin levels of both above- and
underground parts. The contents of cytokinin bioactive forms (free bases and ribosides) were followed. It was revealed that the
growth regulators-modified growth and development as well as the alterations of photosystem II structural organization were related
to the cytokinin levels of Artemisia alba. Thus, elevated monoterpenoid levels were associated with a higher peripheral antennae
aggregation and elevation of trans-zeatin riboside, dihydrozeatin and dihydrozeatin riboside as well as N6-(2-isopentenyl)
adenine in the aerials of the respective plant growth regulators treatments. These results imply of the role of exogenous factors
such as cytokinins and auxins supplementation in affecting the terpenoid biogenesis in vitro by altering the levels of endogenous
cytokinins and physiological status of the plant organism.
Fulltext: contact IEB authors
IEB authors: Petre I. Dobrev,
Václav Motyka
Čeština
English