| 1985 – 1986 | Postdoctoral Fellow, ENEA, C.R.E. Casaccia, Italy |
| 1986 – 1989 | Research Worker, Institute of Experimental Botany, Department of Plant Biotechnology, Olomouc, Czech Republic |
| 1997 – present | Head, Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic |
| 1992 – 2013 | Lecturer/Associated Professor, Palacky University, Department of Cellular Biology and Genetics, Palacky University, Olomouc, Czech Republic |
| 2010 - present | Scientific Director, Centre of the Region Hana for Agricultural and Biotechnological Research, Olomouc |
| 2013 – present | Head , Centre of Plant Structural and Functional Genomics, Olomouc, Czech Republic |
| 2013 - present | Professor of Molecular Biology and Genetics, Department of Cell Biology and Genetics, Faculty of Sciences, Palacky University, Olomouc, Czech Republic |
J. Dolezel served as PI on 19 research projects, including 2 projects from the International Atomic Agency and 1 EU project (INTAS), and as Co-PI on 17 research projects, which included 2 EU projects (COST and 7-th Framework Programme) and 2 US projects (USDA and NSF).
| 1996 – present | Member of Scientific Counsil for Ph.D. studies in Botany, Faculty of Science, Palacky university in Olomouc, Czech Republic |
| 2005 – present | Member of the Management Committee of the Global Musa Genomics Consortium, Montepellier, France |
| 2005 – present | Member of the Coordinating Committee of the International Wheat Genome Sequencing Consortium, USA |
| 2007 – present | Member, Council of the Institute of Biophysics AS CR, v. v. i., Brno, Czech Republic |
| 2007 - present | Elected Member, General Assembly of the Academy of Sciences of the Czech Republic |
| 2008 - present | Member of Committee for the defence of DSc. Theses in Botany and Plant Physiology, the Academy of Sciences of the Czech Republic |
| 2008 - present | Member of Committee for the defence of DSc. Theses in Genetics and Genomics, the Academy of Sciences of the Czech Republic |
| 2012 - present | Member, Council of the Biology Centre, AS CR, v. v. i., Ceske Budejovice, Czech Republic |
| 2014 - present | Member of Scientific Council, Faculty of Science, Palacky University, Olomouc, Czech Republic |
| 2014 - present | Member of the Scientific Council, Mendel University in Brno, Czech Republic |
| 1990 – present | Czech Society for Histochemistry and Cytochemistry (Prague, Czech Republic), member |
| 1997 – present | International Society for Analytical Cytology (Northbrook, USA), member |
| 1999 – present | European Cytogeneticists Association, (Clermont-Ferrand, France), member |
| 2002 – present | Czech Society for Analytical Cytology (Brno, Czech Republic), vice chairman |
| 2005 – present | Genetics Society of America (Bethesda, USA), member |
| 2006 - present | International Society for Horticultural Science (Leuven, Belgium) |
Contributed significantly to the developmnet of methods for analysis of nuclear DNA content, which have been used worldwide in taxonomy, population biology, evolutionary biology and plant breeding. Developed a new approach for plant genomics called 'Chromosomal Genomics', which has been used in the analysis of hereditary information and isolation of genes inplants with large and complex genomes like cereals.
| Lecturer | Three semestral courses: 'Cytogenetics and Cytotaxonomy', 'Genome Anatomy', and 'Cytometric Techniques' at the Department of Cellular Biology and Genetics, Faculty of Science, Palacky University, Olomouc, http://www.upol.cz |
| Bc. students | 3 graduated (currently ongoing 0) |
| M.Sc. students | 6 graduated (currently ongoing 0) |
| Ph.D. students | 12 graduated (currently ongoing4) |
| 1995 – present | Chromosome Research, Kluwer Academic Publishers, Dordrecht |
| 1998 – present | Biologia Plantarum, Institute of Experimental Botany, Prague |
| 2002 – present | Journal of Applied Genetics, Institute of Plant Genetics, Poznan |
| 2006 - present | International Journal of plant Genomics, New York |
| 2008 - present | Genesand Genetic Systems, The Genetics Society of Japan, Mishima |