Molnár, I., Kubaláková, M., Šimková, H., Farkas, A., Cseh, A., Megyeri, M., Vrána, J., Molnár-Láng, M., Doležel, J.
THEORETICAL AND APPLIED GENETICS
127:
1091-1104,
2014
Klíčová slova:
Abstrakt:
The three subgenomes of hexaploid bread wheat
originated from Triticum urartu (AuAu), from a species
similar to Aegilops speltoides (SS) (progenitor of the B
genome), and from Ae. tauschii (DD). Earlier studies indicated
the potential of chromosome genomics to assist gene
transfer from wild relatives of wheat and discover novel
genes for wheat improvement. This study evaluates the
potential of flow cytometric chromosome sorting in the diploid
progenitors of bread wheat. Flow karyotypes obtained
by analysing DAPI-stained chromosomes were characterized
and the contents of the chromosome peaks were determined.
FISH analysis with repetitive DNA probes proved
that chromosomes 5Au, 5S and 5D could be sorted with
purities of 78–90 %, while the remaining chromosomes
could be sorted in groups of three. Twenty-five conserved
orthologous set (COS) markers covering wheat homoeologous
chromosome groups 1–7 were used for PCR
with DNA amplified from flow-sorted chromosomes and
genomic DNA. These assays validated the cytomolecular
results as follows: peak I on flow karyotypes contained
chromosome groups 1, 4 and 6, peak II represented homoeologous
group 5, while peak III consisted of groups 2, 3
and 7. The isolation of individual chromosomes of wild
progenitors provides an attractive opportunity to investigate
the structure and evolution of the polyploid genome and to
deliver tools for wheat improvement.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková,
Jan Vrána
Čeština
English