Analysis of chromosome pairing and recombination
We analyzed chromosome pairing in allohexaploid F. arundinacea, its progenitors F. pratensis and F. glaucescens, and intergeneric hybrids L. multiflorum (2x) × F. arundinacea (6x) and L. multiflorum (4x) × F. glaucescens (4x). To evaluate pairing of individual chromosomes, we analyzed tetraploid lines of L. multiflorum substitution from F. pratensis. GISH permitted the analysis of homoeologous chromosome pairing and recombination of different genomes involved. with monosomic or disomic