Petrovská, B., Jeřábková, H., Kohoutová, L., Cenklová, V., Pochylová, Ž., Gelová, Z., Kočárová, G., Váchová, L., Kurejová, M., Tomaštíková, E., Binarová, P.
JOURNAL OF EXPERIMENTAL BOTANY
64:
4575-4587,
2013
Klíčová slova:
Arabidopsis thaliana, AtTPX2, Aurora kinase, fibres, γ-tubulin, importin, microtubules, nuclei, Ran
Abstrakt:
TPX2 performs multiple roles in microtubule organization. Previously, it was shown that plant AtTPX2 binds AtAurora1
kinase and colocalizes with microtubules in a cell cycle-specific manner. To elucidate the function of TPX2 further, this
work analysed Arabidopsis cells overexpressing AtTPX2-GFP. Distinct arrays of bundled microtubules, decorated with
AtTPX2-GFP, were formed in the vicinity of the nuclear envelope and in the nuclei of overexpressing cells. The microtubular
arrays showed reduced sensitivity to anti-microtubular drugs. TPX2-mediated formation of nuclear/perinuclear
microtubular arrays was not specific for the transition to mitosis and occurred independently of Aurora kinase.
The fibres were not observed in cells with detectable programmed cell death and, in this respect, they differed from
TPX2-dependent microtubular assemblies functioning in mammalian apoptosis. Colocalization and co-purification
data confirmed the interaction of importin with AtTPX2-GFP. In cells with nuclear foci of overexpressed AtTPX2-GFP,
strong nuclear signals for Ran and importin diminished when microtubular arrays were assembled. This observation
suggests that TPX2-mediated microtubule formation might be triggered by a Ran cycle. Collectively, the data suggest
that in the acentrosomal plant cell, in conjunction with importin, overexpressed AtTPX2 reinforces microtubule formation
in the vicinity of chromatin and the nuclear envelope.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Zuzana Gelová,
Hana Jeřábková,
Beáta Petrovská,
Žaneta Pochylová,
Eva Tomaštíková
Čeština
English