Ma, J., Stiller, J., Wei, Y., Zheng, Y.L., Devos, K.M., Doležel, J., Liu, C.
GENOME BIOLOGY AND EVOLUTION
6:
3039-3048,
2014
Keywords:
chromosomal rearrangement, comparative genomics, pericentric inversion, pericentromeric regions, translocation, Chinese Spring
Abstract:
The bread wheat (Triticum aestivum L.) genotype “Chinese Spring” (“CS”) is the reference base in wheat genetics and genomics.
Pericentric rearrangements in this genotype were systematically assessed by analyzing homoeoloci for a set of nonredundant genes
from Brachypodium distachyon, Triticum urartu, and Aegilops tauschii in the CS chromosome shotgun sequence obtained from
individual chromosome arms flow-sorted from CS aneuploid lines. Based on patterns of their homoeologous arm locations, 551
genes indicated the presence of pericentric inversions in at least 10 of the 21 chromosomes. Available data from deletion bin-mapped
expressed sequence tags and geneticmapping in wheat indicated that all inversions had breakpoints in the low-recombinant genepoor
pericentromeric regions. The large number of putative intrachromosomal rearrangements suggests the presence of extensive
structural differences among the three subgenomes, at least some of which likely occurred during the production of the aneuploid
lines of this hexaploid wheat genotype. These differences could have significant implications in wheat genome research where
comparative approaches are used such as in ordering and orientating sequence contigs and in gene cloning.
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IEB authors: Jaroslav Doležel
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