Ouyang, S., Zhang, D., Han, J., Zhao, X., Cui, Y., Song, W., Huo, N., Liang, Y., Xie, J., Wang, Z., Wu, Q., Chen, Y.-X., Lu, P., Zhang, D.-Y., Wang, L., Sun, H., Yang, T., Keeble-Gagnere, G., Appels, R., Doležel, J., Ling, H.-Q., Luo, M., Gu, Y., Sun, Q., Liu, Z.
PLoS ONE
9(6):
e100160,
2014
Keywords:
Abstract:
Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this
study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and
mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was
closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This
suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC
library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing
genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a
fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval.
Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass
species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6,
Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences
were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a
framework for the physical mapping and map-based cloning of MlIW172.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel
Čeština
English