Cerovska N., Moravec T., Hoffmeisterova H., Plchova H., Synkova H., Polakova I., Duskova M., Smahel M.
PLANT CELL, TISSUE AND ORGAN CULTURE
113:
81-90,
2013
Klíčová slova:
Transient expression, Molecular farming, Potato virus X, Human papillomavirus, Oncoprotein E6, Bacterial expression
Abstrakt:
Infection with Human papillomaviruses (HPVs) is linked to cervical cancer, which is one of the most
common cancers found among women worldwide. Despite preventive immunization, a therapeutic vaccine, targeting infected individuals, is required. Vaccine strategies for treatment of HPV—induced cervical cancer are based on E7 and E6 oncoproteins. In this work we report the transient expression of chimeric particles containing the E6 oncoprotein from Human papillomavirus type 16 (HPV-16) in plants. We fused a mutagenized coding sequence of the E6 oncoprotein (E6GT) with the coding sequence of Potato virus X coat protein (PVX CP) both with the 5- and 3-terminus using linkers of different length (0, 4 or 15 amino acids). The expression in E. coli was performed to assess the characteristics of the recombinant protein prior
to plant expression. The yield and immunological reactivity of the expressed proteins were screened with anti-PVX CP and E6 antibodies. The highest yields of chimeric particles were observed in the transgenic N. benthamiana expressing Potato virus A HC-Pro protein and the Tobacco mosaic virus movement protein. When inoculated on host plants, these recombinant viruses were not able to spread systemically. The obtained results revealed the new relations for design of expression cassettes for plant-based vaccine production.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Noemi Čeřovská,
Hana Hoffmeisterová,
Tomas Moravec,
Helena Plchová,
Helena Synková
Čeština
English